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Maria Stella Graziani

Deputy Director
Martina Zaninotto

Associate Editors
Ferruccio Ceriotti
Davide Giavarina
Bruna Lo Sasso
Giampaolo Merlini
Martina Montagnana
Andrea Mosca
Paola Pezzati
Rossella Tomaiuolo
Matteo Vidali

EIC Assistant
Francesco Busardò

International Advisory Board Khosrow Adeli Canada
Sergio Bernardini Italy
Marcello Ciaccio Italy
Eleftherios Diamandis Canada
Philippe Gillery France
Kjell Grankvist Sweden
Hans Jacobs The Netherlands
Eric Kilpatrick UK
Magdalena Krintus Poland
Giuseppe Lippi Italy
Mario Plebani Italy
Sverre Sandberg Norway
Ana-Maria Simundic Croatia
Tommaso Trenti Italy
Cas Weykamp The Netherlands
Maria Willrich USA
Paul Yip Canada

Biomedia srl
Via L. Temolo 4, 20126 Milano

Responsible Editor
Giuseppe Agosta

Editorial Secretary
Chiara Riva
Biomedia srl
Via L. Temolo 4, 20126 Milano
Tel. 0245498282


ISSN print: 0393 – 0564
ISSN digital: 0392- 7091

Biochimica Clinica: VOL.36 N.4

Adiponectin in mother-and-child couple: is there a relation with materno-foetal
F. Ovono Abessolo, J.P. Ngou-Mve-Ngou, J. Mouba, A. Mbang Bengone, E. Ngou-Milama

To evaluate relationships between maternal and fetal adiponectin concentrations and lipoprotein and glycemic parameters in mother-and-child unity at birth, a study was performed with 90 women admitted in the delivery room after a free enlightened consent. Blood samples were taken simultaneously at delivery from mothers (peripheral venous) and newborns (umbilical cord) for glucose, cholesterol, triglyceride, and adiponectin determinations. Multiple correlations between different parameters were made. Adiponectin concentrations in cord blood were three times higher than in the mother’s blood, while total, HDL and LDL cholesterol concentrations were higher in mothers. We demonstrated a slight inverse correlation between maternal adiponectin and gestational age (P=0.026) and between maternal adiponectin and concentrations of triglycerides in mothers (P=0.066). On the other hand, there were positive correlations between adiponectin concentrations, both in mothers and newborns, and LDL cholesterol concentrations in newborns (P=0.0072 and P=0.028, respectively). We inferred that at the end of pregnancy the mother undergoes a situation of insulin resistance, with a decrease in adiponectin concentrations. This may cause the re-routing of her metabolites towards the fetus who under insulin sensitivity would use those metabolites for its metabolic needs. If maternal insulin resistance is higher because of obesity or diabetes, the fetus would receive more metabolites than necessary and would store them as long as its biosynthetic needs are met. This could lead to macrosomia at birth and to obesity afterwards.

Biochimica Clinica 2012; 36(4) 235-239
Contributi Scientifici - Scientific Papers
Preliminary evaluation of a new nephelometric reagent for the determination of serum free light chains
F. Duranti, S. Casciani, P. Casalino, V. Botteri, M.T. Caliò, I. Giambini, S. Bernardini, M. Dessi

The determination of free light chains (FLC) in serum of patients with monoclonal gammopathies is a valuable tool for diagnosis, follow-up, and prognosis of these conditions. The aim of this study was to compare the performance of the FLC reagents from New Scientific Company based on polyclonal antibodies with the Siemens assays (N Latex FLC) based on a mixture of monoclonal antibodies for the nephelometric determination of FLC in serum. Ninety-one serum samples were analyzed for FLC using both assays. Comparisons of the two FLC assays showed a slope of 4.87 (r=0.84) for ĸ FLC determination, a slope of 4.38 (r=0.83) for λ FLC determination, and a slope of 3.42 (r=0.60) for the k/λ FLC ratio. The concordance was 65% for ĸ FLC determinations, 63% for λ FLC, and 71% for k/λ FLC ratio. A preliminary evaluation suggests a higher sensitivity for N Latex FLC assays

Biochimica Clinica 2012; 36(4) 240-243
Contributi Scientifici - Scientific Papers
Indicazioni per la misura delle principali proteine sieriche
Recommendations for the measurement of the most important plasma proteins.
M.S. Graziani, A.Caldini, U. Basile, A. Dolci, S. Ferraro, I. Ferrarotti, I. Infusino, M.T. Muratore, M. Mussap, L. Obici, P. Pezzati, G. Previtali, D. Szőke, A. Terreni, M. Zaninotto, G. Merlini per il Gruppo di Studio SIBioC Proteine

This document, prepared on behalf of the SIBioC Protein Working Group, reviews the existing evidence for recommending the measurement of the most important plasma proteins. For each protein, the related chapter includes: a) a brief introduction describing main biological characteristics, b) the presentation of the available evidence, based on retrieved meta-analyses, systematic reviews, and international and national guidelines, and c) recommendations for the test ordering. The scientific evidence was searched on electronic databases (Medline, Cochrane’s database of systematic reviews) and on the websites of scientific societies. Recommendations were classified on the basis of the source of the retrieved evidence as follows: (+++) official National or International guidelines, (++) meta-analyses or systematic reviews, (±) expert opinions and/or clinical practice, and (–) none (not recommended). The following proteins were considered in the paper: albumin, 1-antitripsin, 1-acid glycoprotein, 2-macroglobulin, haptoglobin, 2-microglobulin, C-reactive protein (both as marker of inflammation and cardiovascular risk), ceruloplasmin, cystatin C, complement factors, rheumatoid factor, ferritin, immunoglobulins (IgA, IgG, IgM, IgD), free light chains, retinol binding protein, soluble transferrin receptor, serum amyloid A, transferrin, and transtyretin.

Biochimica Clinica 2012; 36(4) 244-267
Documenti SIBioC - SIBioC Documents
Genealogia dei metodi d’analisi: l’evoluzione dell’“array”
Analytical Ancestry: Evolution of the Array in Analysis
L.J. Kricka, K. Imai, P. Fortina

Macro-, micro-, and nanosized arrays of test sites at various densities have emerged as important types of analytical devices in response to the need for high volume parallel analysis in both the research and the clinical laboratory. This review explores the  diversity of arrays of reaction vessels and arrays of reagents and of samples, with an emphasis on the earliest descriptions of the different variations. The scope of such  arrays includes linear and 2-dimensional arrays of reaction vessels (e.g., microwell strips, microplates); linear and 2-dimensional arrays of reagents arrayed on pillars and posts; beads in wells; and reagents randomly arrayed (or dis-ordered) for use in next-generation sequencing. Micro- and nanofabrication technologies have been applied to the miniaturization of arrays to increase array density (e.g., DNA probe arrays) and produce arrays of analytical structures (e.g., cantilevers, nanoelectrospray nozzles). Arrays are now firmly established in many types of analytical devices, and this analytical format has gained widespread acceptance owing to the advantages of high-throughput automation and multiplex analysis. Ongoing "big biology" genomic and proteomic studies will ensure the continued dominance of array-based methods into the foreseeable future.

Biochimica Clinica 2012; 36(4) 268-274
Il Meglio di Clinical Chemistry - Clinical Chemistry highlights
Un caso di ipercupremia
A case of hypercupremia
G. Righetti, E. Andreoni, M.G. Anselmi, M. Marini, L. Perobelli, MS Graziani

This report presents a case of an incidental detection of very high concentrations of serum copper in an apparently healthy adult male. Clinical and laboratory  investigations for Wilson’s disease were negative. The electrophoresis of serum proteins showed a IgG 􀀀 monoclonal peak of 10 g/L. To clarify the nature of this unusual association (high concentration of copper and monoclonal immunoglobulin), an immunosubtraction experiment was performed: after incubation with an anti-IgG antiserum, the vast majority of serum copper was found in the IgG-anti IgG complex when the patient serum was tested; when other samples with monoclonal IgG and physiological copper concentrations were incubated, the copper remained in surnatants. The experiment allowed us to establish that the monoclonal immunoglobulin showed a high affinity for copper. Similar reports can be found in the literature; the main difference with the present case is that our patient did not show any ocular problems due to the copper deposition in the Descemet’s membrane, while in all the previously reported cases an ocular deposition of copper was observed. It is possible to speculate that in our case the affinity of the protein for copper was very high, while in other cases a lower affinity made possible the intraocular release of copper.

Biochimica Clinica 2012; 36(4) 275-277
Casi Clinici - Case Report
Esperienza in un presidio antidiabetico ospedaliero con un metodo “point-of-care” per la determinazione dell’emoglobina glicata
Experiences with a POCT system for glycated hemoglobin in a dedicated Diabetes Center
A. Mosca, R. Paleari, C. Bertazzo, A. Lapolla
Biochimica Clinica 2012; 36(4) 278-280
Lettere all'Editore - Letters to the Editor
Bisalbuminemia ereditaria e diabete: quale relazione?
Hereditary bisalbuminemia and diabetes: which relationship?
U. Basile, F. Gulli, E. Di Stasio, M.A. Isgrò
Biochimica Clinica 2012; 36(4) 281
Lettere all'Editore - Letters to the Editor
EuroMedLab 2013 Preliminary Programme
Biochimica Clinica 2012; 36(4) 282-315
- Scientific Meetings
Programma 44° Congresso Nazionale SIBioC
44th SIBioC National Congress Programme
Biochimica Clinica 2012; 36(4) 316-322
- Scientific Meetings
Programma 18° Simposio annuale ELAS Italia
18th ELAS Italia Annual Symposium Programme
Biochimica Clinica 2012; 36(4) 323-326
Convegni e Congressi - Scientific Meetings